lentivirus titer detection kit (Servicebio Inc)
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Lentivirus Titer Detection Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentivirus+titer+detection+kit/concentration+lentivirus+solution/pmc13211383-68-11-16
Average 86 stars, based on 1 article reviews
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1) Product Images from "Chicken lncRNA-9802 Induces the S Phase Arrest in the T Lymphocyte Cells Infected by Marek’s Disease Virus via the TP53BP1/p53/p21 Pathway"
Article Title: Chicken lncRNA-9802 Induces the S Phase Arrest in the T Lymphocyte Cells Infected by Marek’s Disease Virus via the TP53BP1/p53/p21 Pathway
Journal: Veterinary Sciences
doi: 10.3390/vetsci13050469
Figure Legend Snippet: Effect of lncRNA-9802 on the proliferation of MDCC-MSB1 cells assessed by lentivirus-mediated over-expression and knock down. Note: ( A ) MDCC-MSB1 cells were subjected to infection with lentivirus designed for the over-expression (OE) and (KD) of lncRNA-9802 for a duration of 72 h. Successful infection of MDCC-MSB1 cells is indicated by green fluorescence. ( B ) Statistical results of the lentivirus infection efficiency. ( C ) Expression levels of lncRNA-9802 in MDCC-MSB1 cells infected by the lentivirus over-expressing or knocking down for lncRNA-9802. Data are expressed as relative fold changes compared to the negative control (NC) group, following normalization to the endogenous control GAPDH. ( D ) The effect of l ncRNA-9802 on the proliferation of MDCC-MSB1 cells. The independent sample t-test in IBM SPSS Statistics was used to compare differences either between lncRNA-9802 OE NC and lncRNA-9802 OE, or between lncRNA-9802 KD NC and lncRNA-9802 KD. ** represents extremely significant differences between lncRNA-9802 OE NC and lncRNA-9802 OE ( p < 0.01). ## indicates extremely significant differences between lncRNA-9802 KD NC and lncRNA-9802 KD ( p < 0.01).
Techniques Used: Over Expression, Knockdown, Infection, Fluorescence, Expressing, Negative Control, Control
Figure Legend Snippet: The expression levels of genes associated with the p53 pathway following infection with lentivirus for over-expressing and knocking down lncRNA-9802. Note: ( A ) Relative mRNA expression levels of TP53BP1 . ( B ) Relative mRNA expression levels of p53 . ( C ) Relative mRNA expression levels of p21 . ( D ) Representative Western blot images of the TP53BP1/p53/p21 pathway genes in MDCC-MSB1 cells with over-expressed or knocked down lncRNA-9802. ( E ) Relative protein expression levels of TP53BP1. ( F ) The relative protein expression levels of p53. ( G ) Relative protein expression levels of p21. * represents there are significant differences between lncRNA-9802 OE NC and lncRNA-9802 OE ( p < 0.05). ** represents there are extremely significant differences between lncRNA-9802 OE NC and lncRNA-9802 OE ( p < 0.01). # indicates there are significant differences between lncRNA-9802 KD NC and lncRNA-9802 KD ( p < 0.05). ## indicates there are extremely significant differences between lncRNA-9802 KD NC and lncRNA-9802 KD ( p < 0.01). (See ).
Techniques Used: Expressing, Infection, Western Blot
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